| 1 | Up Above My Head by Sister Rosetta Tharpe | 2:31 | |
| 2 | God's Gonna Cut 'Em Down by The Golden Gate Quartet | 2:33 | |
| 3 | I Want My Crown by The Pilgrim Travellers | 2:56 | |
| 4 | Get on Board Little Children by Alphabetical Four | 2:32 | |
| 5 | Climbing up the Mountain by Morris Brown Quartet | 2:21 | |
| 6 | Precious Memories by Sister Rosetta Tharpe | 2:55 | |
| 7 | Didn't It Rain by The Golden Gate Quartet | 2:25 | |
| 8 | Let the Church Roll On by Capital City Four | 2:37 | |
| 9 | Standing on the Highway by The Pilgrim Travellers | 2:15 | |
| 10 | Dig a Little Deeper by Mahalia Jackson | 2:57 | |
| 11 | Oh Mary Don't You Weep by Fisk Jubilee Singers | 1:40 | |
| 12 | Wake Me, Shake Me, Don't Let Me Sleep Too Long by The Wright Brothers Gospel Sin | 2:47 | |
| 13 | Rock Me by Sister Rosetta Tharpe | 2:30 | |
| 14 | I'm Gonna Walk Right in and Make Myself at Home by Alphabetical Four | 2:51 | |
| 15 | Hush by The Golden Gate Quartet | 3:11 | |
| 16 | Where Could I Go but to the Lord by Sister Ernestine Washington | 2:51 | |
| 17 | I Heard Zion by Southern Sons | 2:28 | |
| 18 | Lonesome Road by Sister Rosetta Tharpe | 2:49 | |
| 19 | He Never Said a Mumbling Word by The Golden Gate Quartet | 3:18 | |
| 20 | You've Got to Move by Elder Charles Beck | 2:38 | |
| 21 | Lord I'm Goin Through by Bright Moon Quartet | 3:20 | |
| 22 | Don't Take Everybody to Be Your Friend by Sister Rosetta Tharpe | 2:26 | |
| 23 | The Old Rugged Cross by The Pilgrim Travellers | 2:32 | |
| 24 | Jezebel by The Golden Gate Quartet | 2:47 | |
| 25 | This Heart of Mine by Two Gospel Keys | 2:39 | |
| 26 | Toll the Bell Easy by The Golden Gate Quartet | 2:33 | |
| 27 | Do Lord Send Me by Clara Belle Cholston | 2:48 | |
| 28 | Amazing Grace by Mahalia Jackson | 2:17 | |
| 29 | Everybody's Gonna Have a Wonderful by Sister Rosetta Tharpe | 2:47 | |
| 30 | Dese Bones Gonna Rise Again by The Golden Gate Quartet | 2:16 | |
| 31 | Jesus Hits Like the Atom Bomb by The Pilgrim Travellers | 2:26 | |
| 32 | What Could I Do by Mahalia Jackson | 2:58 | |
| 33 | Lead Me to That Rock by Wandering Boys | 2:49 | |
| 34 | Forgive Me Lord and Try Me One More Time by Sister Rosetta Tharpe | 2:37 | |
| 35 | Swing Down Sweet Chariot by The Golden Gate Quartet | 3:30 | |
| 36 | Go Where I Send Thee by Alphabetical Four | 2:44 | |
| 37 | What a Time Singing with Angels by The Galilee Singers | 2:41 | |
| 38 | What Could I Do by Sister Ernestine Washington | 2:37 | |
| 39 | Shady Green Pastures by The Wright Brothers Gospel Singers | 2:53 | |
| 40 | Jesus Is Here Today by Sister Rosetta Tharpe | 2:49 | |
| 41 | Shadrack by The Golden Gate Quartet | 2:37 | |
| 42 | Fire Down Yonder by Capital City Four | 2:11 | |
| 43 | I'll Tell It by The Pilgrim Travellers | 2:39 | |
| 44 | The Sun Didn't Shine by Alphabetical Four | 2:55 | |
| 45 | Blind Barnabas by The Golden Gate Quartet | 2:08 | |
| 46 | This Train by Sister Rosetta Tharpe | 2:50 | |
| 47 | I See the Sign of Judgement by Bright Moon Quartet | 2:52 | |
| 48 | Satisfied by Wandering Boys | 2:32 | |
| 49 | The Natural Facts (That's All) by Sister Rosetta Tharpe | 3:14 | |
| 50 | Lift Every Voice and Sing by Southern Sons | 2:37 | |
| 51 | Joshua Fit the Battle of Jericho by The Golden Gate Quartet | 2:41 | |
| 52 | Gospel Train by The Wright Brothers Gospel Singers | 2:42 | |
| 53 | My Lord and I by Sister Rosetta Tharpe | 2:48 | |
| 54 | Child of God by William Blevin's Quartette | 2:30 | |
| 55 | High Low and Wide by The Golden Gate Quartet | 2:36 | |
| 56 | There's Not a Friend Like Jesus by Mahalia Jackson | 3:40 | |